Bacterial chromosome extraction and isolation.
basic_science · Level V
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- Record sourced from PubMed, PMID 15100812.
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Abstract
We have used diffusive mixing and dielectrophoretic trapping to lyse Escherichia coli cells in a microfabricated environment and trap the E. coli chromosome. We characterize the conditions needed for efficient lysis of the cells, and conditions needed for the dielectrophoretic trapping of the chromatin without the trapping of cytoplasmic proteins.
Medical subject headings
- Chromosomes, Bacterial