Vesicular glutamate transporters 1 and 2 target to functionally distinct synaptic release sites.

Fremeau, Robert T; Kam, Kaiwen; Qureshi, Tayyaba; Johnson, Juliette; Copenhagen, David R; Storm-Mathisen, Jon; Chaudhry, Farrukh A; Nicoll, Roger A et al. · Science · 2004

basic_science · Level V

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Abstract

Vesicular glutamate transporters (VGLUTs) 1 and 2 show a mutually exclusive distribution in the adult brain that suggests specialization for synapses with different properties of release. Consistent with this distribution, inactivation of the VGLUT1 gene silenced a subset of excitatory neurons in the adult. However, the same cell populations exhibited VGLUT1-independent transmission early in life. Developing hippocampal neurons transiently coexpressed VGLUT2 and VGLUT1 at distinct synaptic sites with different short-term plasticity. The loss of VGLUT1 also reduced the reserve pool of synaptic vesicles. Thus, VGLUT1 plays an unanticipated role in membrane trafficking at the nerve terminal.

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