Ribosomal movement impeded at a pseudoknot required for frameshifting.

Tu, C; Tzeng, T H; Bruenn, J A · Proc Natl Acad Sci U S A · 1992

basic_science · Level V

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Abstract

Translational frameshifting sometimes occurs when ribosomes encounter a "shift" site preceding a region of unusual secondary structure, which in at least three cases is known to be a pseudoknot. We provide evidence that ribosomes have a decreased rate of movement through a pseudoknot required for frameshifting. These paused ribosomes are directly situated over the shift sequence. Ribosomal pausing appears to be necessary but not sufficient for frameshifting.

Medical subject headings