5'-end SAGE for the analysis of transcriptional start sites.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 15300261.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Identification of the mRNA start site is essential in establishing the full-length cDNA sequence of a gene and analyzing its promoter region, which regulates gene expression. Here we describe the development of a 5'-end serial analysis of gene expression (5' SAGE) that can be used to globally identify transcriptional start sites and the frequency of individual mRNAs. Of the 25,684 5' SAGE tags in the HEK293 human cell library, 19,893 matched to the human genome. Among 15,448 tags in one locus of the genome, 85.8%-96.1% of the 5' SAGE tags were assigned within -500 to +200 nt of mRNA start sites using the RefSeq, UniGene and DBTSS databases. This technique should facilitate 5'-end transcriptome analysis in a variety of cells and tissues.
Medical subject headings
- 5' Flanking Region
- Gene Expression Profiling
- Kidney
- Sequence Alignment
- Sequence Analysis, DNA
- Transcription Factors
- Transcription Initiation Site