The inositol polyphosphate 5-phosphatase Ocrl associates with endosomes that are partially coated with clathrin.
basic_science · Level V
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- Record sourced from PubMed, PMID 15353600.
- Also identified by PMC identifier 518786.
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Abstract
The subcellular localization of Ocrl, the inositol polyphosphate 5-phosphatase that is mutated in Lowe syndrome, was investigated by fluorescence microscopy. Ocrl was localized to endosomes and Golgi membranes along with clathrin, giantin, the mannose 6-phosphate receptor, transferrin, and the early endosomal antigen 1 endosomal marker in fixed cells. The endosomal localization of Ocrl was confirmed by live-cell time-lapse microscopy in which we monitored the dynamics of Ocrl on endosomes. GST binding assays show that Ocrl interacts with the clathrin terminal domain and the clathrin adaptor protein AP-2. Our findings suggest a role for Ocrl in endosomal receptor trafficking and sorting.
Medical subject headings
- Clathrin
- Endosomes
- Phosphoric Monoester Hydrolases