Broadband relaxation-optimized polarization transfer in magnetic resonance.

Abstract

Many applications of magnetic resonance are limited by rapid loss of spin coherence caused by large transverse relaxation rates. In NMR of large proteins, increased relaxation losses lead to poor sensitivity of experiments and increased measurement time. In this article, we develop broadband relaxation-optimized pulse sequences that approach fundamental limits of coherence transfer efficiency in the presence of very general relaxation mechanisms that include cross-correlated relaxation. These broadband transfer schemes use techniques of chemical shift refocusing (specific trajectory adapted refocusing echoes) that are tailored to specific trajectories of coupled spin evolution. We present simulations and experimental data indicating significant enhancement in the sensitivity of multidimensional NMR experiments of large molecules through these methods.