"Microcanals" for micropipette access to single cells in microfluidic environments.
basic_science · Level V
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- Record sourced from PubMed, PMID 15472724.
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Abstract
We demonstrate the fabrication and operation of "microcanals"(i.e. open-air microfluidic channels without a roof), which enable micropipette manipulation and probing of cells within a microfluidic environment. The microcanal devices are fabricated in PDMS on glass substrates using a PDMS membrane transferring technique. Here we show patch-clamp electrophysiological recording and intracellular dye injection performed on cells seeded in microcanals.
Medical subject headings
- Microfluidics
- Patch-Clamp Techniques