Requirement for E. coli NusG protein in factor-dependent transcription termination.
basic_science · Level V
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Abstract
The 21 kd NusG protein is essential for E. coli viability. Cells depleted for NusG were defective for factor-dependent transcription termination. Rho-induced polarity in the gal operon and the Rho-dependent lambda tR1 and lambda tL1 terminators were suppressed in NusG-deficient cells. NusG depletion inactivated the phage HK022 Nun termination factor. In contrast, the factor-independent lambda tl terminator was fully active in NusG-depleted cells and could be suppressed by phage lambda N function.
Medical subject headings
- Bacterial Proteins
- Escherichia coli
- Escherichia coli Proteins
- Peptide Elongation Factors
- Transcription Factors