MultiPLX: automatic grouping and evaluation of PCR primers.
other · Level V
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- Record sourced from PubMed, PMID 15598831.
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Abstract
MultiPLX is a new program for automatic grouping of PCR primers. It can use many different parameters to estimate the compatibility of primers, such as primer-primer interactions, primer-product interactions, difference in melting temperatures, difference in product length and the risk of generating alternative products from the template. A unique feature of the MultiPLX is the ability to perform automatic grouping of large number (thousands) of primer pairs. Binaries for Windows, Linux and Solaris are available from http://bioinfo.ebc.ee/download/. A graphical version with limited capabilities can be used through a web interface at http://bioinfo.ebc.ee/multiplx/. The source code of the program is available on request for academic users. maido.remm@ut.ee.
Medical subject headings
- Algorithms
- DNA Primers
- Pattern Recognition, Automated
- Polymerase Chain Reaction
- Sequence Alignment
- Sequence Analysis, DNA
- Software