A rapid method for determining protein kinase phosphorylation specificity.
basic_science · Level V
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Abstract
Selection of target substrates by protein kinases is strongly influenced by the amino acid sequence surrounding the phosphoacceptor site. Identification of the preferred peptide phosphorylation motif for a given kinase permits the production of efficient peptide substrates and greatly simplifies the mapping of phosphorylation sites in protein substrates. Here we describe a combinatorial peptide library method that allows rapid generation of phosphorylation motifs for serine/threonine kinases.
Medical subject headings
- Microfluidic Analytical Techniques
- Peptide Library
- Protein Interaction Mapping
- Protein Serine-Threonine Kinases
- Sequence Analysis, Protein