In vitro transcription of a whole gene on a surface-coupled template.
basic_science · Level V
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- Record sourced from PubMed, PMID 15915259.
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Abstract
An artificial gene was constructed combining the T7 promoter and terminator with the EGFP-gene from the plasmid pEGFP. The functionality of the construct was shown by in vitro translation. The gene-construct was immobilised on a planar glass surface. The transcription was performed on the immobilised gene and mRNA was determined by RT-PCR. Multiple use of the immobilised gene was demonstrated.
Medical subject headings
- Transcription, Genetic