Statistics of protein library construction.
basic_science · Level V
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- Record sourced from PubMed, PMID 15932904.
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Abstract
We have investigated the statistics associated with constructing and sampling large protein-encoding libraries. Using fairly simple statistics we have written algorithms for estimating the diversity in libraries generated by the most commonly used protocols, including error-prone PCR, DNA shuffling, StEP PCR, oligonucleotide-directed randomization, MAX randomization, synthetic shuffling, DHR, ADO and SISDC. Web interface and C++ source code available at http://guinevere.otago.ac.nz/stats.html. Complete mathematical notes, model assumptions and justification, users' guide and worked examples at above website.
Medical subject headings
- Algorithms
- Evolution, Molecular
- Models, Genetic
- Peptide Library
- Polymerase Chain Reaction
- Sequence Analysis, DNA
- Sequence Analysis, Protein
- Software