Restriction mapping in nanofluidic devices.
basic_science · Level V
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- Record sourced from PubMed, PMID 16000405.
- Also identified by PMC identifier 1172226.
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Abstract
We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg2+ and EDTA. We have successfully used the restriction enzymes SmaI, SacI, and PacI, and have been able to measure the positions of restriction sites with a precision of approximately 1.5 kbp in 1 min using single DNA molecules.
Medical subject headings
- DNA
- DNA Restriction Enzymes
- Nanotechnology
- Restriction Mapping