Simple bioseparations using self-cleaving elastin-like polypeptide tags.
basic_science · Level V
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Abstract
We introduce a new method for the purification of recombinant proteins expressed in Escherichia coli using self-cleaving elastin-like polypeptide (ELP) fusion tags without the need for affinity chromatography or proteolytic tag removal. Using this method we obtained high purity, activity and reasonable yields for ten diverse target proteins.
Medical subject headings
- Chemical Fractionation
- Elastin
- Escherichia coli
- Protein Engineering
- Recombinant Fusion Proteins
- Ultrafiltration