Gene expression profiling in single cells within tissue.
basic_science · Level V
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- Record sourced from PubMed, PMID 16118636.
- Also identified by PMC identifier 1976602.
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Abstract
We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.
Medical subject headings
- Biomarkers, Tumor
- Gene Expression Profiling
- In Situ Hybridization, Fluorescence
- Neoplasm Proteins
- Paraffin Embedding
- Prostatic Neoplasms