Defined DNA/nanoparticle conjugates.
basic_science · Level V
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- Record sourced from PubMed, PMID 16155122.
- Also identified by PMC identifier 1200880.
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Abstract
Glutathione monolayer-protected gold clusters were reacted by place exchange with 19- or 20-residue thiolated oligonucleotides. The resulting DNA/nanoparticle conjugates could be separated on the basis of the number of bound oligonucleotides by gel electrophoresis and assembled with one another by DNA-DNA hybridization. This approach overcomes previous limitations of DNA/nanoparticle synthesis and yields conjugates that are precisely defined with respect to both gold and nucleic acid content.
Medical subject headings
- Gold Colloid
- Nanostructures
- Oligonucleotides