Attenuated alloreactivity of dendritic cells engineered with surface-modified microspheres carrying a plasmid encoding interleukin-10.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 16219347.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
In the present study, we investigated MS(O10H6) as a carrier system to introduce a plasmid encoding murine interleukin-10 (pIL-10) to modulate alloreactivity of dendritic cells (DC). Results indicate that MS(O10H6) formed stable and protective nano-sized particles with pIL-10. Gene-modified DC elicited weak proliferation of allogeneic CD4 and CD8 T cells in vitro. Using cell-embedded Matrigel as a surrogate graft, we also showed that DC transfected with MS(O10H6) complexed with pIL-10 suppressed host cell infiltration in vivo. These data demonstrate that the self-assembled system of MS(O10H6) is an effectual delivery vehicle for plasmid-based modulation of DC-dependent allogeneic T cell responses.
Medical subject headings
- Dendritic Cells
- Gene Transfer Techniques
- Histidine
- Interleukin-10
- Microspheres
- Ornithine