In vitro and in vivo two-photon luminescence imaging of single gold nanorods.

Wang, Haifeng; Huff, Terry B; Zweifel, Daniel A; He, Wei; Low, Philip S; Wei, Alexander; Cheng, Ji-Xin · Proc Natl Acad Sci U S A · 2005

basic_science · Level V

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Abstract

Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a cos(4) dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced two-photon absorption cross section. The TPL signal from a single nanorod is 58 times that of the two-photon fluorescence signal from a single rhodamine molecule. The application of gold nanorods as TPL imaging agents is demonstrated by in vivo imaging of single nanorods flowing in mouse ear blood vessels.

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