Arp2/3 complex-deficient mouse fibroblasts are viable and have normal leading-edge actin structure and function.
basic_science · Level V
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- Record sourced from PubMed, PMID 16254049.
- Also identified by PMC identifier 1283463.
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Abstract
RNA interference silencing of up to 90% of Arp3 protein expression, a major subunit of the Arp2/3 complex, proportionately decreases the intracellular motility of Listeria monocytogenes and actin nucleation activity ascribable to the Arp2/3 complex in mouse embryonic fibroblasts. However, the Arp2/3-deficient cells exhibit unimpaired lamellipodial actin network structure, translational locomotion, spreading, actin assembly, and ruffling responses. In addition, Arp3-silenced cells expressing neural Wiskott-Aldrich syndrome protein-derived peptides that inhibit Arp2/3 complex function in wild-type cells retained normal PDGF-induced ruffling. The Arp2/3 complex can be dispensable for leading-edge actin remodeling.
Medical subject headings
- Actin-Related Protein 2-3 Complex
- Actins