Detection of Helicobacter pylori in the middle ear fluid of patients with otitis media with effusion.

Morinaka, Setsuko; Tominaga, Masayuki; Nakamura, Hiroyuki · Otolaryngol Head Neck Surg · 2005

prospective_cohort · Level II

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Abstract

In patients with otitis media with effusion (OME), colonization of the middle ear effusion (MEE) by Helicobacter pylori (HP) was investigated. A prospective nonrandomized study with nonpaired, nonmatched controls. Smear preparations were immunostained with anti-HP antibody and were subjected to Gram staining and Giemsa staining. The rapid urease test (CLO) was done. Twelve of 15 smears for MEE were positive for HP by immunohistochemistry and 14 by Giemsa that were Gram-negative. In 3 with positive immunohistochemistry, the CLO was positive. The results suggest that HP may exist in the MEE of some patients with OME.

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