G-tail telomere HPA: simple measurement of human single-stranded telomeric overhangs.
basic_science · Level V
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Abstract
Accurate measurement of telomeric 3'-overhang (G-tail) lengths is essential for investigation of the biological effects of telomere dysfunction. G-tail telomere hybridization protection assay (Gt-telomere HPA) has the advantages of being simple to perform, accurate and highly sensitive for G tails as short as 20 nucleotides. Furthermore, Gt-telomere HPA is specific and quantitative for human G tails, and can be used to assay cell lysates as well as genomic DNA.
Medical subject headings
- DNA, Single-Stranded
- Nucleic Acid Hybridization
- Telomere