Production of a recombinant bacterial lipoprotein in higher plant chloroplasts.
basic_science · Level V
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Abstract
Little is known about the potential of plastids to accomplish post-translational modifications of foreign proteins. In the present study we generated transplastomic tobacco plants that accumulate the outer surface lipoprotein A (OspA)-the basic constituent of the first generation monovalent human vaccine against Lyme disease. The recombinant OspA exhibits a lipid modification typical for bacteria and induced protective antibodies in mice, demonstrating that functionally active bacterial lipoproteins can be processed in plants.
Medical subject headings
- Antigens, Surface
- Bacterial Outer Membrane Proteins
- Chloroplasts
- Lipoproteins
- Plants, Genetically Modified
- Protein Engineering
- Nicotiana
- Transfection