Protein crystals as scanned probes for recognition atomic force microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 16351189.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Lysozyme crystal growth has been localized at the tip of a conventional silicon nitride cantilever through seeded nucleation. After cross-linking with glutaraldehyde, lysozyme protein crystal tips image gold nanoparticles and grating standards with a resolution comparable to that of conventional tips. Force spectra between the lysozyme crystal tips and surfaces covered with antilysozyme reveal an adhesion force that drops significantly upon blocking with free lysozyme, thus confirming that lysozyme crystal tips can detect molecular recognition interactions.
Medical subject headings
- Crystallization
- Molecular Probe Techniques
- Molecular Probes
- Muramidase
- Nanostructures
- Protein Interaction Mapping