In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes.
basic_science · Level V
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Abstract
MicroRNAs (miRNAs) are 20-23 nucleotide (nt) RNA molecules that regulate gene expression post-transcriptionally. A key step toward understanding the function of the hundreds of miRNAs identified in animals is to determine their expression during development. Here we performed a detailed analysis of conditions for in situ detection of miRNAs in the zebrafish embryo using locked nucleic acid (LNA)-modified DNA probes and report expression patterns for 15 miRNAs in the mouse embryo.
Medical subject headings
- Embryo, Mammalian
- In Situ Hybridization
- MicroRNAs
- Oligonucleotide Probes
- Oligonucleotides, Antisense