Expression of a Wilms tumor gene in porcine kidney during compensatory renal growth.

Abstract

Expression of the putative Wilms tumor gene (WT-1) was studied to investigate its role in renal growth. Compensatory renal growth was induced in 35-day-old Yorkshire-swine by unilateral nephrectomy. The contralateral kidney was removed 0.5 to 72 hours following the initial operation and analyzed for WT-1 gene expression by Northern analysis. Compensatory renal growth was detectable by wet weight measurements at 12 hours following contralateral nephrectomy. WT-1 gene expression was detectable in the contralateral kidney as early as 0.5 hours following unilateral nephrectomy. Expression of this gene was not detected in normal kidney from swine of this age group or from sham operated swine. In addition, we demonstrated the expression of this gene in newborn rat, pig and human kidneys, as well as human fetal kidney, indicating its role in nephrogenesis. These data suggest that expression of the WT-1 gene is involved in the regulatory mechanisms that control different types of renal growth.