Mapping structural interactions using in-cell NMR spectroscopy (STINT-NMR).
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 16432517.
- Also identified by PMC identifier 4447212.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We describe a high-throughput in-cell nuclear magnetic resonance (NMR)-based method for mapping the structural changes that accompany protein-protein interactions (STINT-NMR). The method entails sequentially expressing two (or more) proteins within a single bacterial cell in a time-controlled manner and monitoring the protein interactions using in-cell NMR spectroscopy. The resulting spectra provide a complete titration of the interaction and define structural details of the interacting surfaces at atomic resolution.
Medical subject headings
- Nuclear Magnetic Resonance, Biomolecular
- Protein Interaction Mapping
- Protein Structure, Quaternary