BEAMing up for detection and quantification of rare sequence variants.
basic_science · Level V
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Abstract
BEAMing allows the one-to-one conversion of a population of DNA fragments into a population of beads. We used rolling circle amplification to increase the number of copies bound to such beads by more than 100-fold. This allowed enumeration of mutant and wild-type sequences even when they were present at ratios less than 1:10,000 and was sensitive enough to directly quantify the error rate of DNA polymerases used for PCR.
Medical subject headings
- DNA
- DNA Mutational Analysis
- Nucleic Acid Amplification Techniques