Low stability of Huntington muscle mitochondria against Ca2+ in R6/2 mice.

Gizatullina, Zemfira Z; Lindenberg, Katrin S; Harjes, Phoebe; Chen, Ying; Kosinski, Christoph M; Landwehrmeyer, Bernhard G; Ludolph, Albert C; Striggow, Frank et al. · Ann Neurol · 2006

case_control · Level III

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Abstract

The aim of the present work was the detection of Mitochondrial dysfunction of Huntington's disease (HD). We investigated muscle and muscle mitochondria of 14- to 16-week-old R6/2 mice in comparison with wild-type mice. Atrophic fibers, increased fuchsinophilic aggregates, and reduced cytochrome c oxidase (15%) were found in HD muscle. With swelling measurements and Ca2+ accumulation experiments, a decreased stability of HD mitochondria against Ca2+-induced permeability transition was detected. Complex I-dependent respiration of HD mitochondria was more sensitive to inhibition by adding 10 microm Ca2+ than wild-type mitochondria. Data suggest that the decreased stability of HD mitochondria against Ca2+ contributes to energetic depression and cell atrophy.

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