Multiplex analysis of expression of three IFNbeta-induced genes in antibody-positive MS patients.
case_series · Level IV
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Abstract
Some interferon beta (IFNbeta)-treated patients with multiple sclerosis develop antibody-mediated decreased bioactivity with resultant loss of therapeutic effect. The authors developed real-time multiplex reverse transcriptase PCR to measure expression of three IFNbeta-inducible genes to directly assess IFNbeta bioactivity in patients with neutralizing antibodies (NAbs). The three genes responded in tandem. Correlation of NAb level with bioactivity at low/moderate NAb levels was poor, indicating that for such patients, direct measurement of IFNbeta bioactivity is most reliable.
Medical subject headings
- Adjuvants, Immunologic
- Gene Expression Regulation
- Interferon-beta
- Multiple Sclerosis
- Neutralization Tests