Chromatin remodeling by nucleosome disassembly in vitro.

Lorch, Yahli; Maier-Davis, Barbara; Kornberg, Roger D · Proc Natl Acad Sci U S A · 2006

basic_science · Level V

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Abstract

The RSC chromatin-remodeling complex completely disassembles a nucleosome in the presence of the histone chaperone Nap1 and ATP. Disassembly occurs in a stepwise manner, with the removal of H2A/H2B dimers, followed by the rest of the histones and the release of naked DNA. RSC and related chromatin-remodeling complexes may be responsible for the removal of promoter nucleosomes during transcriptional activation in vivo.

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