Solute convection in dynamically compressed cartilage.

Abstract

Chondrocytes depend upon solute transport within the avascular extracellular matrix of articular cartilage for many of their biological activities. Alterations to solute transport parameters may therefore mediate the cell response to tissue compression. While interstitial solute transport may be supplemented by convection during dynamic tissue compression, matrix compression is also associated with decreased diffusivities. Such trade-offs between increased convection and decreased diffusivities of solutes in dynamically compressed cartilage remain largely unexplored. We measured diffusion and convection coefficients of a wide range of solutes in mature bovine cartilage explant disks subjected to radially unconfined axial ramp compression and release. Solutes included approximately 500 Da fluorophores bearing positive and negative charges, and 10 kDa dextrans bearing positive, neutral, and negative charges. Significantly positive values of convection coefficients were measured for several different solutes. Findings therefore support a role for solute convection in mediating the cartilage biological response to dynamic compression.