Microfluidic system for planar patch clamp electrode arrays.
basic_science · Level V
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Abstract
We present a microfluidic system integrated with disposable cell interface partitions for simultaneous patch clamp recordings. Glass-supported poly(dimethylsiloxane) (PDMS) partitions, having a 2 microm air-blown aperture, were reversibly sealed to a microfluidic system including PDMS channels with isolation valves and microfabricated Ag/AgCl electrodes. Gigaseal recordings from RBL-1 cells were obtained with a 24% success rate. Simultaneous whole cell recordings from valve-isolated electrodes were obtained.
Medical subject headings
- Cell Culture Techniques
- Membrane Potentials
- Microelectrodes
- Microfluidic Analytical Techniques
- Nanotechnology
- Patch-Clamp Techniques