A consensus epitope prediction approach identifies the breadth of murine T(CD8+)-cell responses to vaccinia virus.

Moutaftsi, Magdalini; Peters, Bjoern; Pasquetto, Valerie; Tscharke, David C; Sidney, John; Bui, Huynh-Hoa; Grey, Howard; Sette, Alessandro · Nat Biotechnol · 2006

basic_science · Level V

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Abstract

The value of predictive algorithms for identifying CD8+ T (T(CD8+))-cell epitopes has not been adequately tested experimentally. Here we demonstrate that conventional bioinformatic methods predict the vast majority of T(CD8+)-cell epitopes derived from vaccinia virus WR strain (VACV-WR) in the H-2(b) mouse model. This approach reveals the breadth of T-cell responses to vaccinia, a widely studied murine viral infection model, and may provide a tool for developing comprehensive antigenic maps of any complex pathogen.

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