Sensitive mutation detection in heterogeneous cancer specimens by massively parallel picoliter reactor sequencing.
basic_science · Level V
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Abstract
The sensitivity of conventional DNA sequencing in tumor biopsies is limited by stromal contamination and by genetic heterogeneity within the cancer. Here, we show that microreactor-based pyrosequencing can detect rare cancer-associated sequence variations by independent and parallel sampling of multiple representatives of a given DNA fragment. This technology can thereby facilitate accurate molecular diagnosis of heterogeneous cancer specimens and enable patient selection for targeted cancer therapies.
Medical subject headings
- Chromosome Mapping
- DNA, Neoplasm
- Mutation
- Neoplasms