NSET molecular beacon analysis of hammerhead RNA substrate binding and catalysis.
basic_science · Level V
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Abstract
Nanometal surface energy transfer (NSET), which describes an energy transfer event from optically excited organic fluorophores to small metal nanoparticles, may be used as a molecular beacon/ruler similar to FRET, but with advantages over this classical technique. Here we use NSET to measure Mg(2+)-induced conformational changes for a hammerhead ribozyme and confirm these measurements using FRET. These optical experiments enhance our understanding of the different kinetic pathways for this ribozyme.
Medical subject headings
- Energy Transfer
- Gold
- Nanostructures
- RNA, Catalytic