A global topology map of the Saccharomyces cerevisiae membrane proteome.
basic_science · Level V
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- Record sourced from PubMed, PMID 16847258.
- Also identified by PMC identifier 1544055.
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Abstract
The yeast Saccharomyces cerevisiae is, arguably, the best understood eukaryotic model organism, yet comparatively little is known about its membrane proteome. Here, we report the cloning and expression of 617 S. cerevisiae membrane proteins as fusions to a C-terminal topology reporter and present experimentally constrained topology models for 546 proteins. By homology, the experimental topology information can be extended to approximately 15,000 membrane proteins from 38 fully sequenced eukaryotic genomes.
Medical subject headings
- Proteomics
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins