A genomewide screen for components of the RNAi pathway in Drosophila cultured cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 16882716.
- Also identified by PMC identifier 1525048.
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Abstract
Posttranscriptional silencing by RNAi is initiated by dsRNAs that are processed into siRNAs that ultimately target homologous mRNAs for degradation. We used luciferase reporter constructs and a cultured cell-based assay to perform a genomewide screen for components of the RNAi pathway in Drosophila melanogaster. The screen identified seven genes that affect the RNAi response, five with previously described function (AGO2, Tis11, Hsc70-3, Hsc70-4, and hdc) and two annotated genes (CG17265 and CG10883).
Medical subject headings
- Genome
- RNA Interference