High efficiency gene delivery into laryngeal muscle with bidirectional electroporation.
basic_science · Level V
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- Record sourced from PubMed, PMID 16890069.
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Abstract
The impact of polarity change on the efficiency of in vivo electroporative (EP) gene transfection was assessed in rat laryngeal muscle. High (HV) and low field voltage (LV) were combined with polarity changes to determine transfection in 5 different conditions: 1) without EP (EP[-]), 2) HV+LV (HL), 3) HV+LV followed by HV+LV with no change in polarity (HLHL unidirectional), 4) HV+LV followed by HV+LV with opposite polarity (HLHL bidirectional), 5) HV+LV followed by LV with opposite polarity (HLL bidirectional). HLL bidirectional sequence showed the best result with less interindividual variability and extended expression period. With the exception of repeated high voltage sequences, EP parameters were not likely to induce cell injury or inflammation. HLL bidirectional electroporative gene delivery produces high transfection rates with limited tissue trauma. Bidirectional EP provides a safe and highly efficient method for therapeutic gene delivery into skeletal muscle.
Medical subject headings
- Electroporation
- Laryngeal Muscles
- Transfection