The importance of an endotoxin-free environment during the production of nanoparticles used in medical applications.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 16895356.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We investigated the effect of spherical gold nanoparticles on immature dendritic cells (DCs). Conventionally produced nanoparticles had a maturating effect on the DCs--a result of lipopolysaccharide (LPS) contamination. By modification of the production process, low-LPS particles were obtained, which had practically no effect on phenotypic maturation or cytokine production of the DCs. Our findings emphasize the importance of high purity in the production of nanoparticles, since possible contaminants may interfere with the assessment of biological/medical effects. They also highlight that nanoparticles can function as carriers of immune modulating contaminants.
Medical subject headings
- Dendritic Cells
- Equipment Contamination
- Equipment and Supplies
- Lipopolysaccharides
- Nanostructures