Nanoscale resolution in GFP-based microscopy.
review · Level V
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Abstract
We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The approximately 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.
Medical subject headings
- Endoplasmic Reticulum
- Green Fluorescent Proteins
- Microscopy, Fluorescence
- Microtubules
- Nanovirus