Cystatin C in cerebrospinal fluid and multiple sclerosis.

Hansson, Sara F; Simonsen, Anja Hviid; Zetterberg, Henrik; Andersen, Oluf; Haghighi, Sara; Fagerberg, Inger; Andréasson, Ulf; Westman-Brinkmalm, Ann et al. · Ann Neurol · 2007

case_control · Level III

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Abstract

A recent study using surface-enhanced laser desorption/ionization time-of-flight analysis of cerebrospinal fluid identified a 12.5 kDa truncated isoform of cystatin C (CysC) as a specific biomarker for multiple sclerosis (MS). Surface-enhanced laser desorption/ionization time-of-flight analysis of cerebrospinal fluid samples from 43 MS patients and 46 healthy control subjects. Full-length CysC (13.4 kDa) concentration was similar in MS and control samples. The 12.5 kDa CysC protein was produced from full-length CysC by N-terminal cleavage during storage at -20 degrees C. The 12.5 kDa CysC isoform is a storage-related artifact and is not useful as a diagnostic marker for MS.

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