Characterization of cDNA encoding a human sperm membrane protein related to A4 amyloid protein.
basic_science · Level V
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- Record sourced from PubMed, PMID 1690887.
- Also identified by PMC identifier 53697.
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Abstract
A rat testis lambda gt11 cDNA library was screened with a monoclonal antibody raised against a human sperm membrane protein designated YWK-II. A clone was found with a cDNA insert composed of 1837 base pairs that contained an open reading frame coding for 191 amino acid residues. The deduced polypeptide contained a segment with high homology to the transmembrane-cytoplasmic domains of the A4 amyloid protein found in brain plaques of Alzheimer disease patients. A sequence of basic amino acid residues, Arg-Lys-Arg, was found instead of Lys-Lys-Lys at the probable membrane-cytoplasmic junction that may be a unique property of sperm membrane proteins.
Medical subject headings
- Amyloid
- Amyloid beta-Protein Precursor
- DNA
- Membrane Proteins
- Spermatozoa