The c4 repressors of bacteriophages P1 and P7 are antisense RNAs.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 1696181.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
The c4 repressors of P1 and P7 inhibit antirepressor synthesis and are solely responsible for heteroimmunity of the phages. We show that c4 is a new type of antisense RNA acting on a target, ant mRNA, that is transcribed from the same promoter. Interaction depends on complementarity of two pairs of short sequences encompassing the ribosome binding site involved in ant expression. We demonstrate that heteroimmunity of P1 and P7 is due to just two substitutions in each of the complementary sequences of c4 and ant mRNA. Based on P1-P7 sequence comparison and a mutant analysis, we propose a secondary structure model for c4 RNA, with the complementary regions in loops as important sites for antisense control.
Medical subject headings
- Coliphages
- Escherichia coli
- RNA
- RNA, Messenger