Imaging diacylglycerol dynamics at organelle membranes.
basic_science · Level V
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Abstract
Fluorescence imaging is a powerful technique to visualize spatiotemporal dynamics of biomolecules in living cells. We describe fluorescent indicators for a lipid second messenger, diacylglycerol (DAG), which allow the localized analysis of DAG dynamics at subcellular membranes. We have thus pinpointed that DAG concentrations increase and/or decrease at not only the plasma membrane but also organelle membranes such as endomembranes and mitochondrial outer membranes.
Medical subject headings
- Diglycerides
- Fluorescent Dyes
- Intracellular Membranes
- Mitochondrial Membranes