The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II.
basic_science · Level V
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- Record sourced from PubMed, PMID 17043218.
- Also identified by PMC identifier 1635081.
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Abstract
CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.
Medical subject headings
- Cyclin-Dependent Kinases
- RNA Polymerase II
- Schizosaccharomyces pombe Proteins