Targeting and tracing antigens in live cells with fluorescent nanobodies.
basic_science · Level V
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- Record sourced from PubMed, PMID 17060912.
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Abstract
We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.
Medical subject headings
- Antibodies
- Antigens
- Camelus