Targeted chromosome elimination from ES-somatic hybrid cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 17086180.
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Abstract
To engineer a stem cell genome, we developed a technology for targeted elimination of chromosomes from mouse embryonic stem (ES)-somatic hybrid cells. Here we demonstrate the use of a universal chromosome elimination cassette (CEC) for elimination of a single embryonic stem cell (ESC)-derived chromosome 11 or 12, and also both copies of chromosome 6, which harbor pluripotency-associated genes including Nanog. We attribute hybrid-cell pluripotency to the expression of Nanog from the reprogrammed somatic-cell nuclei.
Medical subject headings
- Chromosome Deletion
- Chromosomes
- Genetic Techniques
- Stem Cells