Sensitive and specific method for detecting G protein-coupled receptor mRNAs.
basic_science · Level V
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Abstract
G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 mug of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.
Medical subject headings
- Oligonucleotide Array Sequence Analysis
- Polymerase Chain Reaction
- RNA, Messenger
- Receptors, G-Protein-Coupled