Comparison of an isoelectric focusing technique and high-performance liquid chromatography for determination of fetal hemoglobin levels.
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Abstract
The authors previously have reported measurements of fetal hemoglobin in infants in blood samples taken at autopsy using an isoelectric focusing (IEF) procedure. The current study was undertaken to compare this methodology with a high-performance liquid chromatography (HPLC) procedure. The correlation coefficient between the IEF and HPLC procedures was 0.938. The HPLC method is technically easier and has fewer disadvantages than the IEF procedure and is recommended for the determination of fetal hemoglobin levels.
Medical subject headings
- Chromatography, High Pressure Liquid
- Fetal Hemoglobin
- Isoelectric Focusing