Sensitive protein detection via triple-binder proximity ligation assays.
other · Level V
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Abstract
The detection of weakly expressed proteins and protein complexes in biological samples represents a fundamental challenge. We have developed a new proximity-ligation strategy named 3PLA that uses three recognition events for the highly specific and sensitive detection of as little as a hundred molecules of the vascular endothelial growth factor (VEGF), the biomarkers troponin I, and prostate-specific antigen (PSA) alone or in complex with an inhibitor--demonstrating the versatility of 3PLA.
Medical subject headings
- Immunoassay
- Molecular Probe Techniques
- Proteins